Fig. 1. Wild type (wt) and mutant ABCA4 express less protein and reduce Hsp27. (A &B) HEK-293 cell lines stably expressing A1038V or G1961E show reduced expression of ABCA4 when compared to wt ABCA4. n=5. (C) Note the profound decrease in steady state levels of Hsp27 by the mutants. (A) Ezrin was used as the loading control. Protein expression of A1038V and G1961E is significantly lower than that of wt ABCA4.